Catalog Number - 68504-05P-2

Cogent RP C4™ is a reversed-phase HPLC stationary phase designed for the separation of hydrophobic and neutral compounds where less retention is desired than with traditional C8 or C18 phases. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this stationary phase provides reproducible chromatographic performance and is widely used as an alternative in USP methods requiring L26 chemistry. Its reduced hydrophobicity compared to longer-chain bonded phases makes it particularly useful for rapid analyses and method development studies.

68504-05P-2 is an HPLC column packed with 5 µm RP C4™ particles featuring a 100 Å pore size in a 2.1 mm ID x 50 mm format. This narrow-bore configuration combines reduced solvent consumption, rapid analysis times, and excellent compatibility with LC-MS instrumentation. The 5 µm particles allow operation at lower backpressure than smaller particle columns while maintaining robust chromatographic performance. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, the HPLC system must be capable of precise and reproducible low-flow operation.

This column is commonly used for hydrophobic and neutral compounds in reversed-phase HPLC when reduced retention is desirable. The C4 bonded phase is often selected for scouting studies, method development, and rapid screening applications where shorter run times and faster equilibration are beneficial. The 2.1 mm ID format is particularly popular for LC-MS workflows due to its low solvent consumption and improved MS compatibility.

Key Benefits

• RP C4™ stationary phase

• USP L26 equivalent chemistry

• Butyl bonded phase

• Fully double end capped

• Ultra-high purity silica support

• Less retention than C8 phases

• Suitable for rapid analyses

• Lower backpressure than smaller particle columns

• 5 µm particle size

• Excellent for LC-MS applications

Typical Applications

• USP L26 methods

• LC-MS methods

• Method development

• Method scouting

• Hydrophobic compound analysis

• Neutral compound analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• High-throughput analysis

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4™

• USP Classification: L26

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for hydrophobic and neutral compounds

• Provides less retention than C8 stationary phases

• Suitable for rapid reversed-phase separations

• The 2.1 mm ID format is ideal for reduced solvent consumption and LC-MS compatibility

• Standard HPLC systems must be capable of reliable operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• The 50 mm length is ideal for short run times and high sample throughput

• 5 µm particles allow higher flow rates with lower backpressure than smaller particle columns

• Fully double end capped to minimize secondary interactions

• Frequently used for method development and scouting studies